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BGI Shenzhen
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GATC Biotech
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BGI Shenzhen
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MGI Tech Co Ltd
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Broad Institute Inc
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CapitalBio Corporation
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GenOne Media Group
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Nextera AS
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Fasteris Life
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BGI Tech Solutions Co Ltd
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Nextera AS
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GenomeScan
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Image Search Results
Journal: PLoS Genetics
Article Title: A Complex Structural Variation on Chromosome 27 Leads to the Ectopic Expression of HOXB8 and the Muffs and Beard Phenotype in Chickens
doi: 10.1371/journal.pgen.1006071
Figure Lengend Snippet: (A) The log 2 fold-change values from whole genome re-sequencing data illustrating the read depth differences between the HB and HQLA breeds. This analysis validates the presence of the CNVs on GGA27 in chickens with the Muffs and beard phenotype that were previously identified using a CGH array experiment. (B) Schematic illustration of the CNV rearrangements in the Mb locus on GGA27. (C) Genes located within the three duplicated CNV regions include the 3’ sequence of PSMC5 and entire SMARCD2 in CNV1 (green shadow), entire HOXB8 and HOXB7 in CNV2 (blue shadow), 5’ sequence of CCR7 , 3’ sequence of KRT222 , and entire SMARCE1 in CNV3 (pink shadow).
Article Snippet:
Techniques: Sequencing
Journal: PLoS Genetics
Article Title: A Complex Structural Variation on Chromosome 27 Leads to the Ectopic Expression of HOXB8 and the Muffs and Beard Phenotype in Chickens
doi: 10.1371/journal.pgen.1006071
Figure Lengend Snippet: The duplicated regions identified by array-CGH are illustrated by green (CNV1), blue (CNV2) and pink (CNV3) boxes, respectively. (A) The boundaries of the CNVs were tested using 8 primers indicated by the arrows located in the known duplicated region of CNV1 and CNV3. All possible amplifications were considered and performed in both Mb and wild-type chickens. (B) The breakpoints of both CNV1_3’ (1,721,521 bp) and CNV3_5’ (4,470,331 bp) were identified after sequencing the specifically-amplified PCR product obtained in Mb chickens using primer F1 and R2. An overlap of two nucleotides was detected in the junction region. Outward facing primers (green and pink arrows) were designed to analyze the other boundary of CNV1 and CNV3. (C) CNV2 was found to be located next to CNV3 in chickens with the Mb phenotype using a genome-walking strategy. The breakpoints of both CNV3_3’ (4,503,417 bp) and CNV2_5’ (3,578,409 bp) were verified by sequencing. A two-nucleotide insertion was found in the junction region. (D) The breakpoints of both CNV2_3’ (3,592,890 bp) and CNV1_5’ (1,702,269 bp) were confirmed through unmapped read alignment of whole genome re-sequencing data. An eight-base insertion was detected in the junction.
Article Snippet:
Techniques: Sequencing, Amplification